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KMID : 0545119980080050461
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Journal of Microbiology and Biotechnology
1998 Volume.8 No. 5 p.461 ~ p.465
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Suspension Culture of an Antibacterial Peptide Producing Cell Line from Bombina orientalis
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Kim, Yong Hwan
Yang, Jae Won/Kim, Chan Wha
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Abstract
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The suspension culture of an anchorage-dependent cell line (Bok-1) from Bombina orientalis was successful in respects of cost and efficiency. The amount of cells obtained from the suspension culture was almost equivalent to that from the anchorage-dependent culture. This result shows the possibility of suspension culture for scale-up. The cells in suspension produced an antibacterial peptide as much as anchorage-dependent cells did. The cell growth (6.0×10 exp (6) cells/㎖) and viability (>80%) at 10 rpm were higher than that at 0 rpm (1.9×10 exp (6) cells/㎖, 65∼80%) and 30 rpm (1.8∼10^6 cells/㎖, 40∼76%). The size of cells became smaller at the agitation rate of 30 rpm. The antibacterial activities of cell extracts from suspension cultured cells were confirmed against gram-negative and gram-positive bacteria by the inhibition zone assay and the liquid growth inhibition assay.
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